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. 2016 Jan 7;17(2):202–219. doi: 10.15252/embr.201540795

Figure EV4. ChAPs influence each others behavior.

Figure EV4

  1. Chs6 with Bud7 but not Bch2 promotes export of Chs3 to the plasma membrane. Cells bearing Chs6 alone or in combination with Bch2 are resistant to calcofluor, while the combination of Chs6 and Bud7 is sensitive to calcofluor. Plates were incubated at 30°C for 2–3 days.
  2. Bch1 requires the presence of at least one other ChAP to be stabilized at the trans‐Golgi network. Binding kinetics of Bch1‐GFP in WT, ΔΔΔ, and ChAP ΔΔ strains. The mean of 20–30 FRAP measurements from different cells is shown. Calculated parameters are shown in Table 2.
  3. Comparison of endogenous (WT) and overexpressed (GPD‐BCH1) Bch1 expression levels in Chs6‐GFP cells. Immunoblot of yeast lysates is shown; Pgk1 serves as a loading control.
  4. Overexpression of Chs6 from GPD‐p426 plasmid in Bch1‐GFP and Chs5‐GFP strains analyzed by immunoblot; Pgk1 serves as a loading control.

Source data are available online for this figure.