Figure 6. ChAPs compete and cooperate for binding to Chs5 in vivo .

• A–D
  ChAPs are differently dependent on each other in terms of binding at the trans‐Golgi network (TGN). Binding kinetics of GFP‐tagged Chs6 (A), Bud7 (B), Bch2 (C), and Bch1 (D) at the TGN in the presence (WT) or absence of the other ChAPs (ΔΔΔ). The mean of 20–30 FRAP measurements from different cells is shown. Calculated parameters are shown in Table 2.