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. 2015 Dec 23;17(2):249–265. doi: 10.15252/embr.201541465

Figure 1. CRISPR/Cas9‐mediated deletion of two GATA motif‐containing intronic sites in Alas2 .

Figure 1

  1. Sequence alignment of GATA and E‐box motifs in Alas2 intron 1 or 8 demonstrating conservation among mammals.
  2. DNase hypersensitivity and ChIP‐seq profiles for GATA‐1 or Pol II occupancy and histone modifications at Alas2 and ALAS2 (accession numbers: GSM912907, GSM912895, GSM1003744, GSM1003753, GSM1014191, GSM923572, GSM946526, GSM935465, and GSM935462).
  3. CRISPR/Cas9 strategy to delete GATA motifs in intron 1 or 8 of Alas2 gene in G1E‐ER‐GATA‐1 cells. PAM: Protospacer adjacent motif.
  4. DNA sequences at Alas2 introns of wild‐type (WT) and mutant clones.