Skip to main content
. 2015 Dec 23;17(2):249–265. doi: 10.15252/embr.201541465

Figure 5. 5‐ALA rescues GATA‐1 activity in Alas2 double‐mutant cells.

Figure 5

  1. Real‐time RT–PCR analysis of mRNA levels of ER‐GATA‐1 target genes and two housekeeping genes, Eif2s1 and Eif3k (n = 4, mean ± SE).
  2. Fold changes of mRNA levels after 5‐ALA treatment relative to no treatment in β‐estradiol‐treated WT clone 1 or double‐mutant clone 1 (n = 4, mean ± SE).
  3. Real‐time RT–PCR analysis of primary transcripts of ER‐GATA‐1 target genes (n = 4, mean ± SE).
  4. Fold changes of primary transcripts with 5‐ALA treatment in comparison with no treatment in β‐estradiol‐treated cells (n = 4, mean ± SE).
Data information: *P < 0.05; **P < 0.01; ***P < 0.001. P‐values were calculated by a two‐tailed unpaired Student's t‐test using Microsoft Excel.