TABLE 1.
PCR primers used in this study
| Target | Primer | Sequence (5′-3′)a | Reference |
|---|---|---|---|
| emm | OM6-16 | GTTTCCTTCATTGGTGCT | 17 |
| OM6-35 | AACAGCAAATTAGCTGCTC | 17 | |
| gusA | gusA-PE | GTTGGGGTTTCTACAGGACG | This study |
| mga | DivMgaL6 | CTTTTAAGAAACTATTTTCC | This study |
| MgaPetI_Nde | ggggcatATGTATGTAAGTAAGTTGTTT | This study | |
| MgaPetII_Xho | aactcgagAGTTGTGGAGGGGG | This study | |
| OYR-4 | GTACCATCAACATTGCG | 19 | |
| Nonspecific | rpsL-1 | GAATGTAGATGCCTACAATTAACCA | 17 |
| rpsL-2 | GTGCGCCACGAACGATATG | 17 | |
| nrdI | nrdI-PE | GGTAATCTGAGGCATAAGTG | This study |
| Pemm6 | Pemm-L1 | GCATGGATCCCATCGCAAAGAGCTTA | 17 |
| Pemm-R1 | GCGGCTCGAGTAGTGTCTATTCGTGTTATT | 17 | |
| Pemm1 | Pemm1-L1 | AAGCGATTATTGACAAGCTC | This study |
| Pemm1-R1 | AGTCAAAGCTACCGCTACTG | This study | |
| Pmga | OYR-25 | GGTTGTACCATAACAGTC | 17 |
| OYL-25 | TACCATAAAATACCTTTC | 17 | |
| PsclA | PsclA-L | TTAAAGAAAGGATCCGGATG | This study |
| PsclA-R | ATGGTGCTTTGATGTCAACA | This study | |
| PsclA-L2 | TGGTAATCGTAATTGTCTGC | This study | |
| PsclA-R2 | GGAGATCAGAGGGCTACTTT | This study | |
| PsclA-L4 | AGCAGTCAAAAAAAGTAACA | This study | |
| PsclA-R4 | TGTACCTTCTTGTCTGGTTG | This study | |
| PsclA-L5 | ACAGCTATGATCATCTATGG | This study | |
| PsclA-R5 | GATTATGGTGCTTTGATGTC | This study | |
| PsclA-L6 | GTGATCCCAAGCCCCAA | This study | |
| PscpA | C5-L1 | AAGAATGAGATTAAGGAGGTCACA | 15 |
| C5-R1 | GCGCAATGGCAAGTTTGTC | 15 | |
| sclA | sclA-F2 | ACGTCGAGTCAAAGAGAGTCAAAAATAAA | This study |
| sclA-R1 | GTTTGGTTAGCTTCTTTGTCA | This study |
a
Underlined nucleotides are restriction sites, and lowercase nucleotides are anchor sequences introduced into the primer.