FIG. 3.
Western blot analysis of HPr synthesis in M. pneumoniae. Antibodies raised against M. pneumoniae HPr were used to determine the total amounts of HPr in cells grown in the presence of glucose (lane 2), glucose and fructose (lane 3), fructose (lane 4), glucose and glycerol (lane 5), or glycerol (lane 6). The concentrations of the carbon sources were 1% (wt/vol). A total of 200 ng of recombinant His6-tagged HPr served as a control (lane 1). His6-tagged HPr is somewhat retarded due to its slightly higher molecular weight.