TABLE 1.
Antibodies used for Western blotting
Primary and secondary antibodies used for Western blotting are listed by manufacturer, catalog number, and lot number. Species of origin as well as antibody specificity to human (H), mouse (M), and rat (R) are also indicated.
| Antibodies | Catalog # | Lot # | Species of origin | Specificity |
|---|---|---|---|---|
| Cell Signaling Technology antibodies | ||||
| AKT-p (Ser-473) | 4060 | 19 | Rabbit | H, M, R |
| AKT | 4691 | 27 | Rabbit | H, M, R |
| JNK-p (Thr-183/Tyr-185) | 9251 | 15 | Rabbit | H, M, R |
| SAPK/JNK | 9252 | 15 | Rabbit | H, M, R |
| c-Jun-p (Ser-63) | 9261 | 14 | Rabbit | H, M, R |
| c-Jun | 9165 | 14 | Rabbit | H, M, R |
| p70S6K1-p (Thr-421/Ser-424) | 9204 | 11 | Rabbit | H, M, R |
| p70S6K1-p (Thr-389) | 9234 | 11 | Rabbit | H, M, R |
| p70S6K1-T | 2708 | 11 | Rabbit | H, M, R |
| rpS6-p(Ser-240/Ser-244) | 5364 | 6 | Rabbit | H, M, R |
| rpS6-p(Ser-235/Ser-236) | 2211 | 11 | Rabbit | H, M, R |
| rpS6 | 2217 | 4 | Rabbit | H, M, R |
| Rictor-p (Tyr-1135) | 3806 | 5 | Rabbit | H, M |
| rictor | 9476 | 5 | Rabbit | H, M |
| eIF4B-p (Ser-422) | 3591 | 6 | Rabbit | H, M, R |
| eIF4B | 3592 | 3 | Rabbit | H, M, R |
| PDCD4 | 9535 | 4 | Rabbit | H, M, R |
| IRS-1-p (Ser-636/639) | 2388 | 14 | Rabbit | H, M, R |
| IRS-1 | 2382 | 4 | Rabbit | H, M, R |
| actin | 3700 | 14 | Mouse | H, M, R |
| Abcam antibodies | ||||
| p70S6K1-p (Ser-229) | ab59208 | GR145615-6 | Rabbit | H, M, R |
| Santa Cruz antibodies | ||||
| GAPDH | 6C5 | K0315 | Mouse | H, M, R |
| α-Tubulin | DM1A | C0112 | Mouse | H, M, R |
| p70S6K1-p (Ser-411) | sc-7983 | K1715 | Rabbit | H, M, R |