FIGURE 6.

The effect of ACTN4 knockdown on dexamethasone-induced GR nuclear translocation in HPCs. A, HPCs were treated with DMSO or dexamethasone (Dex, 100 nm) for 4 h, followed by subcellular fractionation and Western blotting with the indicated antibodies. B, shCtrl and shACTN4 HPCs were treated and analyzed as in A. Only the nuclear fraction is shown. C, shCtrl and shACTN4 HPCs were treated with or without dexamethasone (100 nm) for 1 h, followed by immunostaining with the indicated antibodies, and visualized by fluorescence microscopy. N, nuclear; C, cytoplasmic.