FIGURE 8.

The effect of knocking down HSP90 on the association between ACTN4 and GRα. Knockdown of HSP90 enhances the association of cytoplasmic GR and ACTN4. A, HSP90 was stably knocked down by shRNAs in HPCs. Nuclear (N) and cytoplasmic (C) GR and HSP90 were detected by Western blotting with the indicated antibodies. S.E., shorter exposure; L.S., longer exposure. B, shCtrl and HSP90 knockdown (shHSP90) HPCs were grown and harvested, and nuclear and cytoplasmic extracts were prepared. Cytoplasmic extracts were used for immunoprecipitation (IP) with anti-GR antibodies, and the immunoprecipitates were subjected to Western blotting with the indicated antibodies. Arrowhead, ACTN4 band.