TABLE 2.
Plasmids used in this study
| Plasmid | Relevant genotype or phenotype | Reference |
|---|---|---|
| pAR81 | cat gene from pTP801 ligated into BamHI-digested pTP809; Cmr | This study |
| pAR90 | cfp* inserted between SphI and HindIII sites in pJBA27 | 44 |
| pAR94 | PrrnB, P1 containing SacI-XbaI fragment of pSM1690 inserted into pAR90 digested with SacI and XbaI | This study |
| pAR163 | Amplification of dsred2.T3 with primers Dsred2d and UDsRed2.T3 and ligation into Xbal-HindIII-digested pAR94; results in replacement of cfp* with dsred2.T3, controlled by PrrnB, P1 | This study |
| pAR176 | Amplification of PtetA with primers ar047 and ar048 from pRL27 and ligation into SacI-XbaI-digested pSM1690; results in replacement of PrrnB, P1 with PtetA | This study |
| pAR178 | Amplification of cat gene with primers ar059 and ar060 from pAR81 and ligation with ClaI-SalI-digested p15A ori-containing fragment from pSM1690 (amplified by PCR with primers ar057 and ar058) | This study |
| pAR179 | Insertion of PrrnB, P1-RBSII-dsred2.T3-T0 containing NotI cassette from pAR163 into the NotI site of pAR178 | This study |
| pDsRed.T3 | Encodes a rapidly maturating DsRed.T3 variant | 7 |
| pIB264 | SphI-ClaI aah-aidA-containing fragment from pIB6 | 2 |
| pKKJ128 | flu gene from E. coli MG1655 in pACYC184 | 23 |
| pLH44 | aah-aidA genes from pIB264 ligated into SphI-ClaI-digested pACYC184 | This study |
| pOS32 | gfpmut3b* gene from plasmid pAR176 ligated to XbaI-HindIII-digested pAR179; construct has gfpmut3b* gene under transcriptional control of the E. coli rrnB-P1 promoter | This study |
| pOS33 | aidA gene from pIB264 PCR amplified with primers 482 and 483 and ligated into EcoRV-SalI-digested pACYC184 | This study |
| pOS37 | aah gene from pIB264 PCR amplified with primers 537 and 541 and ligated into BamH1-XmaIII digested pACYC184 | This study |
| pPKL4 | fim gene cluster from E. coli PC31 in pBR322 | 26 |
| pRL27 | mini-Tn5 delivery vector; source of PtetA | 29 |
| pSM1690 | PrrnB, P1-RBSII-gfpmut3b*-T0-T1 NotI fragment in LOW2; Kmr | 49 |
| pTP801 | cat gene in BamHI site of pUC19 | 35 |
| pTP809 | pBR322 derivative with multiple cloning site introduced between EcoRI and NdeI sites | 35 |