FIGURE 3.

MK2/3 signaling promotes TCR-induced mTOR pathway activation in CD4⁺ T cells. A–C, naïve CD4⁺ T cells from the indicated mice were left unstimulated or stimulated with anti-CD3 and anti-CD28. Whole cell lysates were prepared after the indicated durations of stimulation and analyzed by immunoblotting (A). p-, phosphorylated. Immunoblots were scanned and shown in histograms (B). Immunoblot signals were quantified by densitometry, and the relative signal intensities (p-S6 relative to GAPDH) are shown (C). D–G, splenocytes from the indicated mice were treated with anti-CD3 for the indicated durations. Relative p-S6 amounts in CD4⁺ and CD8⁺ cells were analyzed by flow cytometry (D and F). The percentage of p-S6⁺ cells among CD4⁺ and CD8⁺ cells were analyzed by flow cytometry, and is shown as mean ± S.D. (n = 3, each group; E and G). MFI, mean fluorescence intensity. *, p < 0.05; **, p < 0.01. Data are from one experiment (A–C) or representative of three animals with similar results (D–G).