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. 2016 Dec 23;292(5):1762–1772. doi: 10.1074/jbc.M116.764548

FIGURE 4.

FIGURE 4.

T cells lacking p38α and p38β remain capable of TCR-induced activation and differentiation into cytokine-producing effector cells in vitro. A and B, splenic T cells (A) and naïve CD4+ T cells (B) from the indicated mice were labeled with CFSE and stimulated with anti-CD3 and anti-CD28. After 72 h of stimulation, cell proliferation was analyzed by determining the percentage of cells with CFSE dilution among CD4+ cells by flow cytometry. The percentages of divided cells are shown as mean ± S.D. (n = 3, each group). C, naïve CD4+ T cells from the indicated mice were left unstimulated or stimulated with anti-CD3 and anti-CD28 (αCD3/28). After 48 h of stimulation, IFN-γ, IL-13, and IL-17A amounts in the culture supernatants were determined by ELISA, and are shown as mean ± S.D. (n = 2 for IFN-γ and IL-13, each group; n = 3 for IL-17A, each group). D and E, naïve CD4+ T cells from the indicated mice were stimulated with anti-CD3, anti-CD28, and simultaneously treated with varying agents for 5 days to induce Th1 (top), Th2 (middle), and Th17 (bottom) cell differentiation. Effector T cell differentiation was analyzed by determining the percentage of cells with the indicated intracellular cytokines by flow cytometry. Cell percentages are shown as mean ± S.D. (n = 3, each group). Data are from one experiment (A–E).