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. 2016 Dec 23;292(5):1762–1772. doi: 10.1074/jbc.M116.764548

FIGURE 7.

FIGURE 7.

The p38α/β-MK2/3-mTOR signaling module regulates Treg cell induction. A and B, naïve CD4+ T cells from the indicated mice were cultured in a Treg-skewing condition for 5 days as in Fig. 5A. The cells were treated with vehicle (DMSO), PD98059 (PD), and SP600125 (SP) throughout the culture period (A) or without any of these compounds (B). CD25 and Foxp3 expression was analyzed by flow cytometry. C and D, naïve CD4+ T cells from the indicated mice were left unstimulated or stimulated with anti-CD3 and anti-CD28. The cells were left untreated (C) or treated with vehicle (DMSO), the p38 inhibitor SC409 (SC), the MK2 inhibitor PF3644022 (PF), and rapamycin (Rm) 1 h before stimulation (D). Whole cell lysates were prepared after the indicated durations of stimulation and analyzed by immunoblotting. p-, phosphorylated. E, naïve CD4+ T cells were cultured in a Treg-skewing condition for 5 days as in Fig. 5A in the presence of the indicated agents throughout the culture period. CD25 and Foxp3 expression was analyzed by flow cytometry. Data are representative of two (A and E) or three experiments (B–D) with similar results.