FIGURE 2.

RET/PTC3 regulates STAT1 activation. PTC3–5 cell line was incubated with Dox (1 μg/ml) for the indicated times and harvested for cell lysate preparation. Expression of RET, IDO1, pTyr-701 STAT1, pSer-727 STAT1, total STAT1, and tubulin were assayed by immunoblot. RET/PTC3 induced both the up-regulation of STAT1 expression and its full activation through the simultaneous phosphorylation of Tyr-701 and Ser-727. Analysis of IDO1 confirmed RET/PTC3-induced IDO1 overexpression also as protein. All the experiments were repeated twice and the more representative results are depicted.