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. 2016 Dec 20;292(5):1815–1825. doi: 10.1074/jbc.M116.766980

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FIGURE 6. — Pet117 and Cox15 physically interact, and their interaction is not strictly dependent upon Cox15 catalytic activity. A, co-immunoadsorption of differentially tagged Pet117 and Cox15. Digitonin-solubilized mitochondrial lysates from WT or PET117::Myc cells expressing Cox15-FLAG were analyzed as in Fig. 5D. Results are shown for one experiment, representative of three independent experiments (biological replicates). The positions of molecular mass markers are indicated to the left of the immunoblots. B, co-immunoadsorption of differentially tagged Pet117 and a catalytically inactive variant of Cox15. Digitonin-solubilized mitochondrial lysates from cox15Δ or PET117::Myc cox15Δ cells expressing Cox15(H169A)-FLAG were analyzed as in Fig. 5D. Results are shown for one experiment, representative of three independent experiments (biological replicates). The positions of molecular mass markers are indicated to the left of the immunoblots. IP, immunoprecipitation.