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. 2016 Dec 19;292(5):1876–1883. doi: 10.1074/jbc.M116.748707

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Binding of SEFL mAbW variants (mAbW.SEFL1.1, mAbW.SEFL2.0, mAbW.SEFL2.1, and mAbW.SEFL2.2) to human FcγRs. Dose-response binding curves of each mAb to human FcγRI (A), FcγRIIIa (Val¹⁵⁸) (B), FcγRIIa (His¹³¹) (C), and FcγRIIb (D) were generated using FACS cell-based binding assay. mAbW IgG1 and IgG2 were used as controls. Data points were collected in duplicate and the normalized percentage of binding was calculated based on the mAbW.IgG1 for all except hu-FcγRIIa binding, which was based on mAbW.IgG2. The percentage of binding was plotted against antibody concentration.