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. 2016 Dec 19;292(5):1876–1883. doi: 10.1074/jbc.M116.748707

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FIGURE 2. — Binding of SEFL mAbW variants to cynomolgus monkey FcγRs. Dose-response binding curves of each mAb to cynomolgus monkey FcγRI (A), FcγRIIIa (B), FcγRIIa (C), and FcγRIIb (D) were generated using FACS cell-based binding assay. mAbW.IgG1 and mAbW.IgG2 antibodies were used as controls. Data points were collected in duplicate and the normalized percentage of binding was calculated based on mAbW.IgG1 for all except cy-FcγRIIa binding. The percentage of binding was plotted against antibody concentration.