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. 2016 Dec 19;292(5):1876–1883. doi: 10.1074/jbc.M116.748707

FIGURE 6.

FIGURE 6.

Phagocytosis of platelets by cynomolgus monocytes in vitro. Cynomolgus peripheral blood leukocytes and CMFDA-labeled platelets were co-incubated with a test article for 6 h and assessed for phagocytosis of platelets by monocytes by measuring CMFDA fluorescence in monocytes by flow cytometry. A, cynomolgus peripheral blood leukocytes and CMFDA-labeled platelets were co-incubated with mAbY.IgG2, mAbY.IgG1, or mAbY.SEFL2.0. Dotted line indicates the MFI of the IgG2 isotype control at the lowest concentration tested. B, cynomolgus peripheral blood leukocytes and CMFDA-labeled platelets were co-incubated with mAbW.IgG1, mAbW.SEFL1.1, mAbW.SEFL2.0, mAbW.SEFL2.1, or mAbW.SEFL2.2. Note that different antibodies and instruments were used in A and B and this results in the baseline variation. However, it is clear that SEFL antibodies do not induce phagocytosis unlike the IgG1 or IgG2 controls.