TABLE 2.
Analysis of biofilm development in wild-type and Pf4-expressing P. aeruginosa strains with the COMSTAT softwarea
| Parameter | Day | Wild type | Wild type + Pf4 | SCV7 |
|---|---|---|---|---|
| Mean biomass (μm−3 μ−2) | 1 | 0.43 ± 0.09 | 0.30 ± 0.02 | 0.47 ± 0.13 |
| 3 | 0.74 ± 0.07 | 0.94 ± 0.13 | 1.19 ± 0.55 | |
| 7 | 1.87 ± 0.07 | 2.48 ± 0.57 | 5.29 ± 0.62 | |
| Mean thickness (μm) | 1 | 0.36 ± 0.09 | 0.32 ± 0.03 | 0.53 ± 0.14 |
| 3 | 0.82 ± 0.06 | 1.01 ± 0.11 | 1.41 ± 0.65 | |
| 7 | 2.02 ± 0.03 | 3.08 ± 0.89 | 6.37 ± 0.93 | |
| Maximum thickness (μm) | 1 | 11.40 ± 1.53 | 11.9 ± 0.33 | 41.60 ± 1.60 |
| 3 | 11.66 ± 0.21 | 15.2 ± 0.02 | 43.20 ± 9.73 | |
| 7 | 12.80 ± 0.32 | 19.2 ± 0.89 | 57.60 ± 7.82 | |
| Mean microcolony area at | 1 | 9.73 ± 2.93 | 5.28 ± 2.36 | 123.49 ± 51.62 |
| substratum (μm2) | 3 | 5.76 ± 5.75 | 8.11 ± 3.61 | 181.31 ± 81.62 |
| 7 | 17.00 ± 2.95 | 26.93 ± 4.64 | 294.70 ± 146.22 |
a
The values are means ± standard errors for data from 15 image stacks (five image stacks from three replicate biofilms) Boldface type indicates values that are significantly higher than the values for the wild-type strain as determined by analysis of variance.