FIGURE 2.

ICAP1_FL and ICAP1_PTB bind integrin β1 tails equally well. A, pull-down of GFP-tagged ICAP1 constructs from CHO cell lysates with purified recombinant αIIb or β1 integrin tails. Tail loading was assessed by Coomassie Blue staining. The input lane indicates 5% of input lysate. B, ICAP1 binding to purified recombinant αIIb and β1 tails was quantified and expressed as a percentage of input (mean ± S.E. (error bars); n = 4). C, increasing amounts of purified ICAP1_FL or ICAP1_PTB were pulled down with His-tagged integrin tails (either αIIb or β1a) immobilized on beads. Protein was detected by immunoblot analysis. D, a binding curve was generated by quantifying the bands, normalizing to the input control, and plotting the relative signal versus the input of purified protein (mean ± S.D. (error bars), n = 3).