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. 2016 Dec 21;292(5):1884–1898. doi: 10.1074/jbc.M116.762393

FIGURE 4.

FIGURE 4.

ICAP1 contains a functional NLS. A, CHO cells overexpressing GFP-tagged ICAP1 constructs were plated on fibronectin, fixed 24 h later, and stained with DAPI to identify nuclei. Representative images are shown; bar, 10 μm. B, relative amount of GFP intensity in the nucleus compared with the integrated GFP intensity of the entire cell. Boxes, 25th through 50th and 50th through 75th percentile; whiskers, 5th through 95th percentile (n = 88–139 cells from 5 independent experiments). *, p ≤ 0.005 as determined by a one-way ANOVA with Tukey's correction for multiple tests. C, representative fractionation of CHO cells overexpressing GFP-tagged ICAP1 constructs. C, 28% of the cytoplasmic fraction; N, 80.0% of the nuclear fraction. Carbonyl reductase (CBR1) and histone deacetylase (HDAC1) represent quality controls for cytoplasmic and nuclear fractions, respectively. D, quantification of cell fractionation data where the percentage nuclear = total nuclear/(total nuclear N + total cytoplasmic) × 100 (bar, mean percentage nuclear value; n = 9). *, p ≤ 0.02 as determined by a one-way ANOVA with Tukey's correction for multiple tests.