Localization of KRIT1Nterm is driven by
ICAP1.
A and B, CHO cells
co-overexpressing GFP-tagged KRIT1 constructs and DsRed-tagged
ICAP1 constructs were plated on fibronectin, fixed 24 h later,
and stained with DAPI to identify nuclei. Images were analyzed
using CellProfiler. Percentage nuclear fluorescence compared
with the total integrated fluorescence intensity of the entire
cell for DsRed (A) and GFP (B)
was calculated. Boxes, 25th through 50th and
50th through 75th percentile; whiskers, 5th
through 95th percentile (n = 62–138
cells from 3 independent experiments). *, p
≤ 0.0001 as determined by a one-way ANOVA with Tukey's
correction for multiple tests.