FIGURE 7.

Evaluation of putative KRIT1 localization sequences. A, schematic of KRIT1 noting the sequence of NLS1, NES, and NLS2 as well as domain boundaries. B, CHO cells overexpressing GFP-tagged KRIT1 constructs were plated on fibronectin, fixed 24 h later, and stained with DAPI to identify nuclei. Representative images are shown; bar, 10 μm. C, relative amount of GFP intensity in the nucleus compared with the integrated GFP intensity of the entire cell. Boxes, 25th through 50th and 50th through 75th percentile; whiskers, 5th through 95th percentile (n = 85–157 cells from 4 independent experiments). *, p ≤ 0.0001 as determined by a one-way ANOVA with Tukey's correction for multiple tests.