FIGURE 4.

Expression profiles of relevant genes involved in Fgf16 signaling, iBAT activation, bile acid metabolism, and lipid absorption. Two weeks post-AAV injection, iWAT (A), eWAT (B), liver (C), ileum (D), and pancreas (E) tissue from EV mice (red bars) and FGF16 mice (blue bars) were collected and analyzed for gene expression changes. Data are presented as the average relative mRNA ± S.E. Statistical significance was determined using an unpaired Student's t test, correcting for multiple comparisons using the Holm-Sidak method with α = 5.000%. *, p < 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001.