FIG. 5.

Genetically distinct A. marginale strains differ in reactivity with a MAb specific for the appendage-associated protein. The Virginia (V), Illinois (I), and Florida (F) strains were examined by phase-contrast (Phase) and fluorescence (AnaO24D5) microscopy of the same field labeled with MAb AnaO24D5. Replicates with MAb AnaO23A5 gave the same result (not shown). Omission of the MAb (control) or use of an unrelated MAb was used as a negative control for nonspecific immunofluorescence. The white bar in the upper left corner of panel V represents 10 μm.