TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant comment(s) | Source or reference |
|---|---|---|
| Strains | ||
| Y. pestis | ||
| KIM5-3001 | Spontaneous Strr mutant of KIM5 (Pgm−), psaA+ | 23 |
| KIM5-4001 | pCD1− from KIM5-3001, Strr | This study |
| KIM5-3001.1 | psaA mutant of KIM5-3001, Chlr | |
| KIM5-4002 | pCD1− from KIM5-3001.1, Chlr | This study |
| KIM5-4003 | KIM5-3001.1 with pDG5, Chlr Ampr | This study |
| KIM5-4004 | KIM5-3001.1 with pIC20R, Chlr Ampr | This study |
| KIM5-4005 | KIM5-4002 with pDG5, Chlr Ampr | This study |
| KIM5-4006 | KIM5-4002 with pIC20R, Chlr Ampr | This study |
| E. coli | ||
| DH5α | Laboratory stock | |
| HB101 | Laboratory stock | |
| DH5α pUT | Kanr | Laboratory stock |
| Plasmids | ||
| pIC20R | pUC-like plasmid with more restriction sites, Ampr, 9.4-kb ClaI fragment with psaA cloned into pIC20R | 24 |
| pDG5 | 24 |