TABLE 2.
Comparison of phagocytosis of Y. pestis KIM5 isogenic strains grown in different media by RAW264.7 mouse macrophages
| Y. pestis strain | Phagocytosis (%)a
|
|
|---|---|---|
| Growth in pH 6 MgOX SBHI | Growth in pH 7 MgOX BHI | |
| KIM5-3001 (pCD1+psaA+) | 2.8 ± 2.8* | 0.08 ± 0.07 |
| KIM5-4001 (pCD1−psaA+) | 5.1 ± 3.1 | 2.1 ± 1.8 |
| KIM5-3001.1 (pCD1+psaA mutant) | 22.1 ± 17.0 | 0.37 ± 0.38 |
| KIM5-4002 (pCD1−psaA mutant) | 24.4 ± 19 | 9 ± 2.9 |
| KIM5-4003 (pDG5 pCD1+psaA mutant) | 0.27 ± 0.15 | NDb |
| KIM5-4005 (pDG5 pCD1−psaA mutant) | 0.33 ± 0.34 | ND |
| KIM5-4004 (pIC20R pCD1+psaA mutant) | 10.3 ± 7.8 | ND |
| KIM5-4006 (pIC20R pCD1−psaA mutant) | 12.4 ± 3.4 | ND |
a
Values represent means ± standard deviations for three or four experiments each with duplicate samples.
b
ND, not determined