TABLE 3.
Numbers of Y. pestis KIM5 Isogenic bacteria of phagocytosed by and adherent to RAW264.7 macrophages as determined by fluorescence microscopy
| Strain | No. of extracellular bacteriaa | No. of intracellular bacteriaa | Total no. of extra- and intracellular bacteriab |
|---|---|---|---|
| KIM5-3001 (pCD1+psaA+) | 115.4 ± 27.3 | 65.6 ± 16.5 | 181.0 ± 43.8 |
| KIM5-3001.1 (pCD1+psaA mutant) | 70.0 ± 9.1 | 129.0 ± 22.0 | 199.0 ± 31.1 |
a
Extracellular bacteria imaged as red-orange (labeled with rhodamine and Fluorescein isothiocyanate [FITO]), while intracellular bacteria imaged as green (labeled with FITC) (see Materials and Methods and Fig. 6). Values represent means ± standard deviations for five fields of bacteria in the semiconfluent mouse macrophage monolayer under a fluorescence microscope.
b
Values represent means ± standard deviations of total numbers of extra- and intracellular bacteria described in footnote a.