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. 2016 Dec 29;173(2):1075–1093. doi: 10.1104/pp.16.01790

Figure 8.

Figure 8.

Effects of recombinant AtPMEI4 and AtPMEI9 on PMEs from root cell wall-enriched protein extracts. A, Recombinant AtPMEI4 and AtPMEI9 show distinct inhibitory capacities and pH dependence on PMEs from 10-d-old root cell wall-enriched protein extracts. Experiments were carried out using 1 milliunit of PME activity of this extract at pH 5 (left), pH 6.3 (middle), and pH 7.5 (right) and various quantities of purified PMEIs: AtPMEI4 (black bars), AtPMEI9 (gray bars), and AcPMEI (white bars). Controls represent extract with boiled PMEI. Results are means ± sd of three replicates. B, Effects of AtPMEI4 (left) and AtPMEI9 (right) on PME activity from wild-type (black bars) and atpme3 (white bars) root total protein extracts at pH 5. Experiments were carried out using 1 milliunit of PME activity of these extracts at pH 5, and the addition of AtPMEI4 and AtPMEI9 at different concentrations was performed. Experiments were performed in triplicate.