Figure 5.

Identification of binding sites for Tam3 TPase-interacting factors for detaining Tam3 TPase at the PM. A, Amino acid sequences of Znf-BED domains in the 10 constructs (m1 to m10) carrying point mutations and wild-type (wt) are depicted. Each construct was fused with the GFP gene at C termini. The gray background represent conserved sites; amino acid positions 186 and 189 for the N-terminal aromatic amino acids; 200, 203, 221, and 226 for the zinc finger. Dashes represent the same amino acid as wild-type. B, Subcellular localization of point mutation constructs. Ten constructs were transformed into protoplasts of Antirrhinum. Proportion of the cells with GFP in the PM to the total number of cells with GFP were counted under a fluorescence microscope (Supplemental Table S3). Data represent means ± sd.