Subcellular localization of GET factors in Arabidopsis. A, Extracts from Arabidopsis seedlings expressing GET1-YFP, GET3-YFP, and GET4-YFP were fractionated into microsomal and cytoplasmic fractions. Immunoblot of proteins obtained from the two fractions was probed with anti-GFP, antitubulin (a cytoplasmic marker), and anti-BiP (an ER marker) antibodies. B and C, Confocal microscopy of N. benthamiana leaf epidermal cells agroinfiltrated with constructs expressing GET1-YFP (B) and GET3-YFP (C), both with the ER marker CDC-960 mCherry. Bar = 10 µm.