Skip to main content
. 2016 Dec 15;173(2):1434–1452. doi: 10.1104/pp.16.01734

Figure 2.

Figure 2.

Reversible inhibition of HDAC activity by GSNO and SNAP in protoplasts and nuclear extracts. A, HDAC activity after GSNO and SNAP treatments in protoplasts. Water was used as a solvent control. B, DTT restored HDAC activity after GSNO and SNAP treatments. Protoplasts were first incubated with GSNO and SNAP before the addition of DTT. Subsequently, HDAC activity was measured. C, cPTIO prevented the inhibition of HDACs by GSNO. Protoplasts were preincubated with cPTIO for 5 min before the addition of GSNO, and HDAC activity was recorded. D and E, HDAC activity after GSNO and SNAP treatment in nuclear extracts from suspension cells (D) and liquid-grown seedlings (E). Nuclear extracts were treated with water (solvent control), GSH (control), GSNO, or SNAP for 20 min at room temperature in the dark. After desalting, HDAC activity was measured. F and G, DTT restored HDAC activity after SNAP and GSNO treatments. Nuclear extracts were first incubated with GSNO and SNAP before the addition of DTT. After desalting, HDAC activity was determined. Values are normalized to untreated nuclear extracts or protoplasts. Values shown are means ± se of three independent preparations of nuclear extract or protoplasts. *, P < 0.05; **, P < 0.01; and ***, P < 0.001 by Student’s t test.