Figure 4.

AvrPtoB interacts specifically with tomato group III E2 members in vivo. BiFC was used to detect the interaction of AvrPtoB and group III E2s. Different construct pairs for the BiFC assay were transiently coexpressed in N. benthamiana leaves. Leaves were viewed with the confocal microscope to detect chlorophyll autofluorescence (red color) and green fluorescence (YFP). The control vector expressing N-terminal YFP-fused AvrPtoB_1-307 (AvrPtoB_1-307-nYFP) and C-terminal YFP-fused SlUBC16 (SlUBC16-cYFP) were used as negative controls for E3 and E2, respectively. The experiment was repeated two times with similar results. Bars = 20 μm.