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. 2016 Dec 1;173(2):1371–1390. doi: 10.1104/pp.16.01190

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Figure 6. — Knocking down the group III ubiquitin E2 genes down-regulates the induction of PTI-activated reporter genes by flg22. The expression of the N. benthamiana PTI reporter genes Wrky28, Pti5, Gras2, and Acre31 in 2 μm flg22-treated leaves of group III E2 gene-silenced and nonsilenced TRV control N. benthamiana plants was analyzed by real-time PCR at the indicated time points after flg22 treatment. The x axis of each plot marks different time points (hours) after flg22 treatment. The experiment was performed with three technical repeats in each of the three biological replicates. Error bars indicate sd. Asterisks mark significant reductions of the expression of PTI reporter genes in group III E2 gene-silenced plants compared with nonsilenced TRV control plants (P < 0.05).