Figure 3.
A, The levels of FLC mRNA in FRI_Col (WT) and transgenic plants during vernalization. B, The levels of FT mRNA in FRI_Col (WT) and transgenic plants during vernalization. C, The levels of FLC clade genes, FLM and MAF2 to MAF5, in FRI_Col (WT) and transgenic plants during vernalization. Statistically significant changes are indicated by asterisks (Student’s t test, P < 0.01; n = 3). N.S., Not significant. A to C, PP2A (At1g13320) is used as a control for qRT-PCR analyses. Data (relative levels; mean ± sd of quantitative RT-PCR; n = 3). NV, Nonvernalized; 20V, 20 d of vernalization; 40V, 40 d of vernalization; 40VT10, 40 d of vernalization followed by 10 d of normal growth temperature.