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. 2004 Dec;72(12):7367–7373. doi: 10.1128/IAI.72.12.7367-7373.2004

FIG. 2.

FIG. 2.

CTxβ-mediated patching of GM1 does not impair C. trachomatis infection. HeLa cells were subjected to CTxβ-mediated patching of GM1 and infected with the indicated C. trachomatis serovar. (Upper) The effect of patching 1 h postinfection was revealed by IF microscopy using fluorescent-conjugated CTxβ. (Lower) At 20 h postinfection, the total number of inclusions was determined by IF microscopy for 10 fields. The fraction of control cells infected with C. trachomatis was set to 100%, and the fraction of infected cells following patching was normalized to this value. Error bars represent the standard deviations for an experiment done in triplicate and did not reach statistical significance compared to the control sample by Student's two-tailed unpaired t test.