Figure 1.
pkl shows a reduced response to GA. A and B, ga1-13 pkl is less responsive to GA than ga1-13. Plants were grown in the presence of different concentrations of GA4 for 7 d under continuous light. A, Scale bar, 2 mm. B, Average hypocotyl lengths are shown as means ± se (n = 10). *P < 0.05; **P < 0.01 (Student’s t test). C, PKL-myc and RGA interact in vivo. Immunoprecipitation was performed using anti-myc antibody and protein extracts of wild-type (WT) and PPKL:PKL-myc pkl1-1 seedlings that were grown in the presence of PAC. Coimmunoprecipitated RGA was detected using anti-RGA antibody. *Nonspecific band. D, Immunoblot shows comparable accumulation of RGA proteins in ga1 pkl and ga1 seedlings grown as in A.