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. 2016 Dec 20;173(2):1453–1462. doi: 10.1104/pp.16.01514

Figure 3.

Figure 3.

Auxin-independent elongation of GFP-AtSAUR19 hypocotyl segments. A, Elongation of 5-mm auxin-depleted Arabidopsis hypocotyl segments on medium ±10 μm IAA. B, Elongation of 8-mm auxin-depleted tomato hypocotyl segments on medium ±10 μm IAA. C, Elongation of auxin-depleted Arabidopsis hypocotyl segments on medium ±10 μm IAA. For Arabidopsis assays, hypocotyl segments were prepared from 3- to 4-d-old etiolated Col and GFP-AtSAUR19 seedlings. For the D1-OX line, however, seedlings were grown for 11 d in order to obtain hypocotyl segments that were long enough to conduct the assay. Tomato hypocotyl segments were excised from 6-d-old etiolated seedlings. All data points represent the mean relative segment length ± se from a minimum of five hypocotyls/genotype/treatment. All assays were repeated at least three times. D, qRT-PCR analysis of auxin-depleted Arabidopsis hypocotyl segments treated with 10 μm IAA. Values indicate the mean relative expression (±sd) from three biological replicates.