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. 2016 Dec 2;173(2):944–955. doi: 10.1104/pp.16.01527

Figure 4.

Figure 4.

Expression profiles of WSL4. A, qPCR analysis of WSL4 mRNA expression. Lb, Leaf blade; St, stem; Inf, inflorescence; Sh, sheath; Ap, aerial part of 2-week-old seedling; R, root from 2-week-old seedling. Data are means ± se of three biological replicates. B, Spatial expression pattern of the WSL4 gene in transgenic rice plants harboring the WSL4 promoter fused to the GUS gene. a, Germinating seed; b, spikelet; c, anther; d, root; e, young leaf; f, leaf sheath; g, cross section of stem; h, cross section of leaf sheath; i, cross section of anther; j, cross section of young leaf; and k, cross section of root. Bars = 100 μm (h–k). C, Subcellular localization of WSL4. The green and magenta signals obtained with confocal microscopy indicated fusion proteins WSL4-GFP and mCherry-HDEL (ER marker protein), respectively. The overlap of GFP and mCherry fluorescent signals is indicated in merged images. Bars = 10 μm.