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. 2016 Dec 16;173(2):1330–1341. doi: 10.1104/pp.16.01594

Figure 1.

Figure 1.

Transport capability and energy coupling of SbSUTs after expression of their cDNAs in heterologous systems. A, Time course of Suc accumulation from a 1-mm Suc solution by the yeast strain SEY6210 transformed with SbSUT1, SbSUT4, SbSUT5, or empty pDR195 vector. B, Time course uptake from a 15-mm Suc solution by SEY6210 transformed with SbSUT4 or pDR195 empty vector. Uptake experiments were carried out at pH 5. Mean ± sd (vertical bars) of three biological replicates. C, D, and E, Current traces of X. laevis oocytes injected with (C) SbSUT1, (D) SbSUT5, or (E) uninjected, when exposed to a modified Na-Ringer solution at pH 5.6 containing 10 mm Suc. Traces shown are of single oocytes. Scale in E also applies to C and D. F, Changes in Suc uptake (expressed as proportion of control) by yeast transformed with SbSUT1 and SbSUT5 in the presence of the protonophores CCCP (10 µm) and DNP (50 µm) or the sulfhydryl reagent DEPC (1.5 mm). Mean ± sd (vertical bars) of three biological replicates.