Table I. Comparisons of the expression levels of suberin and cutin biosynthetic and deposition-related genes in the wild type (Col-0) and myb107, showing the fold change of gene expression in myb107 versus the wild type quantified by RNA-seq and qRT-PCR.
For repeat 1 and repeat 2 of the RNA-seq and qRT-PCR experiments, RNAs from mixed siliques of different growth stages were used. For repeat 3 of the qRT-PCR experiment, RNAs from stage III developing seeds were used. Fold change values less than 0.5 are highlighted in boldface. ND, Not determined.
| Gene Name | Arabidopsis Genome Initiative No. | Pathways |
RNA-Seq (myb107/Wild Type) |
qRT-PCR (myb107/Wild Type) |
|||
|---|---|---|---|---|---|---|---|
| Repeat 1 | Repeat 2 | Repeat 1 | Repeat 2 | Repeat 3 | |||
| BAHD acyltransferase | |||||||
| HHT/ASFT | AT5G41040 | Suberin | 0.59 | 0.48 | 0.48 | 0.63 | 0.15 |
| FACT | AT5G63560 | Suberin | 0.10 | 0.11 | 0.39 | 0.39 | 0.06 |
| DCF | AT3G48720 | Cutin | 0.95 | 0.59 | 1.18 | 1.74 | 0.73 |
| DCR | AT5G23940 | Cutin | 1.15 | 1.17 | 0.99 | 0.80 | 1.02 |
| Cytochrome P450 monooxygenase | |||||||
| CYP86A1 | AT5G58860 | Suberin | 0.49 | 0.17 | 0.49 | 0.57 | 0.59 |
| CYP86B1 | AT5G23190 | Suberin | 0.38 | 0.46 | 0.39 | 0.32 | 0.25 |
| CYP86A2 | AT4G00360 | Cutin | 0.89 | 0.87 | 0.76 | 0.76 | 0.63 |
| CYP86A8 | AT2G45970 | Cutin | 0.79 | 0.71 | 0.85 | 0.60 | 0.65 |
| CYP77A6 | AT3G10570 | Cutin | 1.16 | 0.92 | 1.22 | 0.60 | 1.23 |
| Long-chain acyl-CoA synthetase | |||||||
| LACS1 | AT2G47240 | Cutin | 1.00 | 0.92 | ND | ND | ND |
| LACS2 | AT1G49430 | Cutin/suberin | 0.57 | 0.62 | 0.40 | 0.45 | 0.49 |
| Glycerol-3-phosphate acyltransferase | |||||||
| GPAT5 | AT3G11430 | Suberin | 0.84 | 0.60 | 0.68 | 1.12 | 0.36 |
| GPAT4 | AT1G01610 | Cutin/suberin | 0.95 | 0.49 | 0.71 | 0.43 | 0.45 |
| GPAT6 | AT2G38110 | Cutin | 0.84 | 1.02 | 0.83 | 0.63 | 1.02 |
| GPAT8 | AT4G00400 | Cutin | 0.91 | 1.64 | 0.98 | 0.83 | ND |
| Alcohol-forming fatty acyl-CoA reductase | |||||||
| FAR1 | AT5G22500 | Suberin | 0.33 | 0.53 | 0.20 | 0.37 | 0.08 |
| FAR4 | AT3G44540 | Suberin | 0.24 | 0.23 | 0.28 | 0.36 | 0.15 |
| FAR5 | AT3G44550 | Suberin | 0.57 | 0.59 | 0.36 | 0.67 | 0.28 |
| 3-Ketoacyl-CoA synthase | |||||||
| KCS2 | AT1G04220 | Wax/suberin | 1.00 | 0.85 | 0.78 | 0.73 | 1.06 |
| KCS20 | AT5G43760 | Wax/suberin | 0.82 | 0.79 | 0.81 | 0.70 | 0.58 |
| ABC transporter | |||||||
| ABCG2 | AT2G37360 | Suberin | 0.53 | 0.72 | ND | ND | ND |
| ABCG6 | AT5G13580 | Suberin | 1.55 | 1.21 | 0.93 | 0.65 | 0.86 |
| ABCG20 | AT3G53510 | Suberin | 0.86 | 0.80 | 0.56 | 0.58 | 0.84 |
| ABCG11 | AT1G17840 | Cutin | 0.92 | 1.02 | 1.43 | 0.68 | 0.64 |