Figure 8.

Ultrastructure of the cuticle and localization of HG in the cell wall-cuticle continuum in different double mutants having low amounts of 10,16-diOH C16:0. The cell wall-cuticle continuum on the adaxial side of the petal at the apex of epidermal cells is shown. The ultrastructure of the cuticle was depicted in A, C, E, and G on sections embedded in Spurr’s resin. The LM20 labeling of esterified HG is depicted on LR White-embedded sections in B, D, and F. CP, Cuticle proper; Cu, cuticle; CW, cell wall, xCM, extracuticular material. Bars = 100 nm in A to F and 250 nm in G.