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Quantitative PCR analysis of GA biosynthetic and signaling protein genes from imbibed atslp2-2, wild-type NÖ, AtSLP2pro::AtSLP2, and 35Spro::AtSLP2 seeds. Seeds were imbibed for 0 h, 6 h, and 12 h in water at 4°C in the dark followed by snap freezing. A to F, Transcriptional expression of Arabidopsis SLP2, MIA40, GA3 oxidase (GA3OX), GA-insensitive dwarf 1a (GID1A), regulator of GA1 (RGA1), and regulator of GA-like 2 (RGL2) was assessed. Error bars represent ± se (n = 3).
