. 2016 Dec 30;173(2):1094–1108. doi: 10.1104/pp.16.01675

Table II. Calculated and native masses of complex II subunits and subcomplexes in Arabidopsis.

n.i., Not identified.

Subunit	Accession No.^a	Calculated Mass Precursor^b	Calculated Mass Mature^b^,^c	Apparent Mass^d	Holo Complex II	Subcomplex IIa	Subcomplex IIb	Subcomplex IIc
		kD	kD	kD
SDH1	At5g66760	69.656	66.043	65	+	+
SDH1	At2g18450	69.363	65.745		+	+
SDH2	At3g27380	31.171	28.253	29	+	+
	At5g40650	31.141	28.143
	At5g65165	34.983	n.i.
SDH3	At5g09600	23.454	12.172	12	+		+
SDH3	At4g32210	23.454	12.172		+		+
SDH4	At2g46505	16.842	9.181	6	+		+
SDH5	At1g47420	28.106	18.417	18	+			+
SDH6	At1g08480	15.813	15.682	15	+		+
SDH7	At3g47833	10.298	7.095	7	+		+
SDH7	At5g62575	10.860	7.485		+		+
SDH8	At2g46390	4.884	4.884	5	+		n.i.
Σ calculated mass (kD)^e			161.7		161.7	94.3	44.1 + SDH8^f	18.4
Σ apparent mass (kD)^e				157	157	94	40 + SDH8^f	18

Accession numbers according to TAIR (https://www.arabidopsis.org/). Note that two isoforms are present for SDH1, SDH3, and SDH7 and three isoforms are present for SDH2. ^bMolecular masses were calculated using the ProtParam tool (http://web.expasy.org/protparam/). ^cFor definition of the mature sequences, see Supplemental Figure S4. ^dApparent molecular masses as determined by Millar et al. (2004). ^eMolecular masses for the holo complex and subcomplexes IIa, IIb, and IIc were determined considering their subunit compositions. Subunit masses were summed using the first isoform given in this table. ^fNote that inclusion of SDH8 in subcomplex IIb is not clear.