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Expression of AidA is controlled by the cep QS system. The B. cenocepacia wild-type strain H111 and various isogenic mutants were grown in LB medium overnight. Total-cell proteins were separated by SDS-PAGE, and AidA was visualized by immunoblotting with antisera directed against the protein. (a) Lanes: 1, H111; 2, H111-I (cepI); 3, H111-I (cepI) plus 200 nM C8-HSL; 4, H111-R (cepR); 5, H111-R(pBAH27). (b) Lanes: 1, H111; 2, H111-A (aidA); 3, H111-A(pBAH44); 4, H111-A(pBAH62).
