FIG. 5.

Expression of AidA is growth phase dependent. The B. cenocepacia wild-type strain H111 was grown in LB medium, and samples were taken along the growth curve. AHL concentrations were quantified by mixing 10 μl of cell-free culture supernatant with 100 μl of an exponentially growing culture of the GFP-based AHL biosensor P. putida(pAS-C8). Following incubation for 4 h, relative fluorescence units (rfu) were measured with a fluorimeter (a). Expression of AidA was assessed by normalizing samples to an OD₆₀₀ of 1 and using 10 μl for Western blotting with anti-AidA antibodies (b).