Table 1. PCR Primers Used in the Gene Expression Analysis.
| Gene name | Description | Sequence (5′-3′) | Length (bps) |
|---|---|---|---|
| β-actin | mβ-actin - F | CTGTCCCTGTATGCCTCTG | 221 |
| mβ-actin - R | TTGATGTCACGCACGATT | ||
| P38 | mP38 - F | GGAGAAGATGCTCGTTTTGGA | 211 |
| mP38 - R | TTGGTCAAGGGGTGGTGG | ||
| JNK | mJNK - F | TCTCCAGCACCCATACATCAA | 150 |
| mJNK - R | TCCTCCAAATCCATTACCTCC | ||
| Nrf-2 | mNrf-2 - F | TCCTATGCGTGAATCCCAAT | 103 |
| mNrf-2 - R | GCGGCTTGAATGTTTGTCTT | ||
| Keap1 | mKeap1 - F | GATCGGCTGCACTGAACTG | 106 |
| mKeap1 - R | GGACTCGCAGCGTACGTT | ||
| NF-κB | mNF-κB - F | GGTGGAGGCATGTTCGGTA | 142 |
| mNF-κB - R | TGACCCCTGCGTTGGATT | ||
| NQO1 | mNQO1 - F | TTCTGTGGCTTCCAGGTCTT | 104 |
| mNQO1 - R | TCCAGACGTTTCTTCCATCC | ||
| GCLC | mGCLC - F | ATGTGGACACCCGATGCAGTATT | 200 |
| mGCLC - R | GTCTTGCTTGTAGTCAGGATGGTTT | ||
| HO-1 | mHO-1 - F | GACAGAAGAGGCTAAGACCGC | 213 |
| mHO-1 - R | TGGAGGAGCGGTGTCTGG |
Note: The primers were manufactured by Nanjing Genescript Co. Ltd. (Nanjing, PR China). P38, p38 MAP Kinase; JNK, c-Jun N-terminal protein kinase; Nrf-2, NF-E2-related factor 2; Keap1, Kelch-like ECH-associated protein1; NF-κB, nuclear factor κB; NQO1, NAD(P) H: quinoneoxidoreductase; HO-1, heme oxygenase 1; GCLC, catalytic subunit of glutamate-cysteine ligase.