Figure 4. In vitro validation of transcription factor activated lentiviral reporter vectors.

(A) Schematic representation of parental lentiviral reporter construct. (B–E) 293 T cells were transduced with VSV-G pseudotyped TFAR lentiviral vectors at an MOI of 10 then incubated with the relevant agonists and luciferase expression quantified at 12, 24, 48 and 72 h (n = 3–6, mean+/− SEM, comparison by analysis of variance with repeated measures).