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. 2017 Feb 3;7:41874. doi: 10.1038/srep41874

Table 2. Primary and secondary antibodies used for immunohistochemistry.

Antibody Supplier Antigen Retrieval Dilution
Chicken αGFP NEB (13970) Na Citrate (pH6) 1:300
Mouse αCK7 Perseus (PP-H415-00) Na Citrate (pH6) 1:200
Mouse αHNF-4α Dako (M0851) Tris HCl (pH10) 1:100
Rabbit αSMA Dako (Z0334) Na Citrate (pH6) 1:200
Rabbit αGFAP Dako (M7018) Tris HCl (pH10) 1:500
Rabbit α-PKM2 Life Technologies (PA5-23034) Na Citrate (pH6) 1:200
Goat α-rabbit Alexafluor Life Technologies (A-11011)   1:500
Goat α-mouse Alexafluor Life Technologies (A-11031)   1:500
Goat α-chicken Alexafluor R&D Systems (NL018)   1:400
DAPI Sigma Aldrich (D9542)   1:1000