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Effect of early post-ischemic reperfusion and tissue plasminogen activator (tPA) on 3-nitrotyrosine levels (3-NT) and smooth muscle F-actin in middle cerebral arteries (MCAs). Smooth muscle 3-NT and filamentous (F)-actin were not affected by early post-ischemic reperfusion. (a) Representative images of MCAs from all groups of animals and (b) quantification of smooth muscle 3-NT and F-actin in all groups of animals. 3-NT and F-actin content of MCAs were not significantly affected by MCAO
